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The strategy for digital PCR (dPCR) has been summarized as and conquer a sample is diluted and partitioned into hundreds or even millions of separate reaction chambers so that each contains one or no copies of the sequence of interest. By counting the number of partitions (in which the sequence is detected) versus partitions (in which it is not), scientists can determine exactly how many copies of a DNA molecule were in the original sample. Among other applications, researchers have used digital PCR to distinguish differential expression of alleles1, to track which viruses infect individual bacterial cells2, to quantify cancer genes in patient specimens3 and to detect fetal DNA in circulating blood4..

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